FastQCFastQC Report
Wed 5 Jul 2017
FCHGVKNBBXX-HKZEBggcRAABRAAPEI-202_L3_1.fq

Summary

[OK]Basic Statistics

MeasureValue
FilenameFCHGVKNBBXX-HKZEBggcRAABRAAPEI-202_L3_1.fq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36926312
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG54450.018.2606644
AATCGGT54850.017.846765
TTACCCG65700.017.2176692
TACCCGA66050.016.9852833
AGAATCG58350.016.9270363
ACGCGTA44100.016.56071322
CTTACCC82250.016.3920861
CCCGACG77350.014.5039365
CACGCGT52600.013.92641121
CCACGCG53700.013.68210420
ACGATCC39950.013.3252083
CGCGTAT61400.013.1175691
CGGCGAT52600.012.9209321
ATCGGTT80600.012.8001146
CCGACGC88800.012.7328666
TCGGTTT82850.012.1869827
TGCCGTA64500.011.76692631
CTCGGAT75300.011.4580491
GCGTATC72250.011.331352
CGACAAT48000.011.3089631
Produced by FastQC (version 0.11.5)